8QKS
Plasmodium falciparum reticulocyte-binding protein homologue 5 (PfRH5) bound to R5.034
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 82.259, 376.787, 226.818 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 65.604 - 3.994 |
| Rwork | 0.441 |
| R-free | 0.45810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.711 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.604 | 4.060 |
| High resolution limit [Å] | 3.990 | 3.990 |
| Number of reflections | 115243 | 5241 |
| <I/σ(I)> | 0.319 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.993 | 0.220 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.02 M Calcium chloride dihydrate 0.02 M Cadmium chloride hydrate 0.02 M Cobalt(II) chloride hexahydrate 20% w/v Polyethylene glycol 3,350 |
