8QKS
Plasmodium falciparum reticulocyte-binding protein homologue 5 (PfRH5) bound to R5.034
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 82.259, 376.787, 226.818 |
Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
Resolution | 65.604 - 3.994 |
Rwork | 0.441 |
R-free | 0.45810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.711 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.604 | 4.060 |
High resolution limit [Å] | 3.990 | 3.990 |
Number of reflections | 115243 | 5241 |
<I/σ(I)> | 0.319 | |
Completeness [%] | 99.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.993 | 0.220 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.02 M Calcium chloride dihydrate 0.02 M Cadmium chloride hydrate 0.02 M Cobalt(II) chloride hexahydrate 20% w/v Polyethylene glycol 3,350 |