8QKR
Plasmodium falciparum reticulocyte-binding protein homologue 5 (PfRH5) bound to R5.251
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-03 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 |
Unit cell lengths | 69.927, 92.016, 92.492 |
Unit cell angles | 60.53, 72.13, 79.82 |
Refinement procedure
Resolution | 59.104 - 3.234 |
Rwork | 0.254 |
R-free | 0.32680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.397 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.104 | 3.290 |
High resolution limit [Å] | 3.130 | 3.230 |
Number of reflections | 30110 | 5486 |
<I/σ(I)> | 0.405 | |
Completeness [%] | 98.6 | |
Redundancy | 3.7 | |
CC(1/2) | 0.955 | 0.245 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.3M Calcium chloride 0.1M Magnesium formate HCl 22.5% (v/v) PurePEGs Cocktail |