8QKR
Plasmodium falciparum reticulocyte-binding protein homologue 5 (PfRH5) bound to R5.251
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-03 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.927, 92.016, 92.492 |
| Unit cell angles | 60.53, 72.13, 79.82 |
Refinement procedure
| Resolution | 59.104 - 3.234 |
| Rwork | 0.254 |
| R-free | 0.32680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.397 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.104 | 3.290 |
| High resolution limit [Å] | 3.130 | 3.230 |
| Number of reflections | 30110 | 5486 |
| <I/σ(I)> | 0.405 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.955 | 0.245 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.3M Calcium chloride 0.1M Magnesium formate HCl 22.5% (v/v) PurePEGs Cocktail |
