8QJK

Structure of the cytoplasmic domain of csx23 from Vibrio cholera in complex with cyclic tetra-adenylate (cA4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9537
Spacegroup name	I 4
Unit cell lengths	66.425, 66.425, 42.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.970 - 1.761
Rwork	0.243
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.112
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0350)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.970	1.910
High resolution limit [Å]	1.760	1.760
Number of reflections	5251	264
<I/σ(I)>	14.3
Completeness [%]	89.1
Redundancy	13.3
CC(1/2)	1.000	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.2MLiS04, 0.1M NaAcetate, 42.5% peg400