8QJ3
Receptor Sd-Amt1 (OFF-state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | H 3 |
| Unit cell lengths | 114.757, 114.757, 278.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.970 - 1.900 |
| R-factor | 0.16332 |
| Rwork | 0.162 |
| R-free | 0.18440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.610 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 1.351 |
| Rmeas | 0.080 | 1.487 |
| Rpim | 0.033 | 0.621 |
| Total number of observations | 624924 | 29533 |
| Number of reflections | 107695 | 5208 |
| <I/σ(I)> | 14.8 | 1.4 |
| Completeness [%] | 99.8 | |
| Redundancy | 5.8 | 5.7 |
| CC(1/2) | 0.999 | 0.404 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis/Tris pH 6.5 with 25 % (w/v) PEG 3350 |
