8QI8
Cryogenic temperature dark state structure of CrPhotLOV1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 121.090, 121.090, 46.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.640 - 1.350 |
| R-factor | 0.1268 |
| Rwork | 0.126 |
| R-free | 0.14820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.640 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.052 | 2.239 |
| Number of reflections | 43943 | 4076 |
| <I/σ(I)> | 30.1 | |
| Completeness [%] | 99.4 | |
| Redundancy | 19.29 | |
| CC(1/2) | 1.000 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM Na cacodylate pH6.5 1 M Na citrate dibasic trihydrate |
