8QHK
Crystal structure of reduced respiratory Complex I subunits NuoEF from Aquifex aeolicus bound to reduced 3-acetylpyridine adenine dinucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-24 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.413, 116.013, 189.741 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.538 - 1.950 |
Rwork | 0.167 |
R-free | 0.20030 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.137 |
Data reduction software | autoPROC |
Data scaling software | Aimless (0.7.9) |
Phasing software | REFMAC (5.8.0352) |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.538 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rpim | 0.035 | 0.436 |
Number of reflections | 102689 | 5013 |
<I/σ(I)> | 15.7 | 2 |
Completeness [%] | 99.9 | |
Redundancy | 13.3 | |
CC(1/2) | 0.999 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | Tris, BisTris, trisodium citrate, ammonium sulfate, NaCl |