8QHF

Corynebacterium glutamicum mycoloyltransferase C acyl-enzyme intermediate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98013
Spacegroup name	F 2 3
Unit cell lengths	168.120, 168.120, 168.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.530 - 2.690
R-factor	0.2545
Rwork	0.253
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4 (10-JUL-2024))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	2.850
High resolution limit [Å]	2.688	2.690
Rmeas	0.198	2.449
Number of reflections	11045	1723
<I/σ(I)>	12.21
Completeness [%]	99.6
Redundancy	9.7
CC(1/2)	0.997	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	Ammonium sulfate, Sodium chloride, Hepes