8QH7
Crystal structure of respiratory Complex I subunits NuoEF from Aquifex aeolicus bound to reduced 3-acetylpyridine adenine dinucleotide (without reducing agent)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.233, 116.449, 190.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.695 - 1.850 |
| Rwork | 0.171 |
| R-free | 0.20060 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.165 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC (5.8.0352) |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rpim | 0.039 | 0.421 |
| Number of reflections | 120438 | 5883 |
| <I/σ(I)> | 13.6 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.5 | 13.3 |
| CC(1/2) | 0.999 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | trisodium citrate, ammonium sulfate, sodium chloride, Tris, BisTris |
