8QGR

Stopper protein of phage 812 in closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 6 2 2
Unit cell lengths	93.366, 93.366, 122.531
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.159 - 2.200
Rwork	0.190
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.223
Data reduction software	XDS (2016-11-01)
Data scaling software	Aimless
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.200	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.130	0.689
Number of reflections	16664	1627
<I/σ(I)>	23.26	4.21
Completeness [%]	99.9	99.8
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	1.6 M NaCl, 8% PEG 6000, 20% glycerol, 3% dextran sulphate sodium