8QF3
1.25-A structure of anti-Arc nanobody H11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.170, 57.170, 145.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.430 - 1.250 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.070 | 3.278 |
| Rmeas | 0.075 | 3.717 |
| Number of reflections | 127672 | 12759 |
| <I/σ(I)> | 9.5 | 0.5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.1 | |
| CC(1/2) | 0.999 | 0.188 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 281 | 0.1 M Bis-Tris (pH 5.5) and 25% w/v PEG 3350 |
