8QDO

Crystal structure of the tegument protein UL82 (pp71) from Human Cytomegalovirus

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978565
Spacegroup name	I 21 21 21
Unit cell lengths	90.610, 137.440, 275.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.610 - 2.700
R-factor	0.2079
Rwork	0.207
R-free	0.23060
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.701
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.614	2.800
High resolution limit [Å]	2.700	2.700
Rmeas	0.075	1.209
Number of reflections	47593	4846
<I/σ(I)>	22.55	2.11
Completeness [%]	100.0	100
Redundancy	13.76	14.4
CC(1/2)	0.999	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES pH 6.5, 0.2 M MgCl2, 7.25% w/v PEG4000
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES pH 6.5, 0.2 M MgCl2, 7.25% w/v PEG4000