8QCL
A carbohydrate esterase family 15 (CE15) glucuronoyl esterase from Phocaeicola vulgatus ATCC 8482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-09 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9117 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.282, 59.525, 87.452 |
| Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
| Resolution | 48.930 - 1.840 |
| R-factor | 0.17528 |
| Rwork | 0.172 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.458 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.930 | 1.999 |
| High resolution limit [Å] | 1.800 | 1.838 |
| Number of reflections | 42976 | 2149 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 94.8 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.996 | 0.575 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.0, 20 mM NaCl, and 20-24% (w/v) PEG 6000 |
