8QCI

FCGBP D10 Assembly Segment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-22
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.34
Spacegroup name	P 21 21 21
Unit cell lengths	43.716, 54.321, 129.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.490 - 2.200
R-factor	0.2005
Rwork	0.196
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.897
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.490	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.165	0.897
Rpim	0.067	0.509
Number of reflections	16209	1567
<I/σ(I)>	10.9	2.3
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.988	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	50 mM Tris, pH 8.0, 22% w/v PEG 8,000, 20% v/v glycerol