8QC9
Crystal structure of the outer membrane decaheme cytochrome MtrC (A293Boc-Lys)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-11 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.078, 90.021, 154.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.630 - 2.000 |
R-factor | 0.1752 |
Rwork | 0.173 |
R-free | 0.21430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.096 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.630 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.346 | |
Number of reflections | 50997 | 3730 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.4 | 13.9 |
CC(1/2) | 0.991 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 200 mM Na acetate pH 5.0, 100 mM CaCl2 and 21% PEG 6000 |