8QB6

Dispersin from Terribacillus saccharophilus DispTs2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	89.688, 89.688, 97.685
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.398 - 1.510
Rwork	0.153
R-free	0.17840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.593
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.400	30.400	1.540
High resolution limit [Å]	1.510	8.270	1.510
Rmerge	0.056	0.033	1.764
Rmeas	0.062	0.037	1.966
Rpim	0.027	0.017	0.860
Number of reflections	71635	492	3522
<I/σ(I)>	17.7
Completeness [%]	100.0
Redundancy	10	8.4	10
CC(1/2)	0.999	0.998	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	MPD screen E7: 0.1 M Citric acid pH 4.0, 20% MPD