8QB4

Crystal structure of apo-GltTk obtained with in meso crystallization (H32 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97242
Spacegroup name	H 3 2
Unit cell lengths	92.850, 92.850, 335.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.880 - 2.700
R-factor	0.2558
Rwork	0.254
R-free	0.29760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.395
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.770
High resolution limit [Å]	2.700	2.700
Rmeas	0.340	4.649
Number of reflections	15646	1538
<I/σ(I)>	8.28	0.69
Completeness [%]	99.2	100
Redundancy	19.3	20.1
CC(1/2)	0.997	0.201

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	1.6 M ammonium phosphate