8QAX

Medicago truncatula HISN5 (IGPD) in complex with MN, FMT, GOL and TRS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9763
Spacegroup name	I 4
Unit cell lengths	120.587, 120.587, 182.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.290 - 1.690
R-factor	0.1718
Rwork	0.172
R-free	0.20020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.790
High resolution limit [Å]	1.690	1.690
Rmerge	0.053	2.031
Rmeas	0.056	2.150
Number of reflections	145061	23408
<I/σ(I)>	21.62	1.1
Completeness [%]	99.9	99.2
Redundancy	9.1
CC(1/2)	0.999	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	2.0 M sodium formate, 0.1 M sodium acetate