8QAW

Medicago truncatula HISN5 (IGPD) in complex with MN, IMD, EDO, FMT, GOL and TRS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	H 3
Unit cell lengths	137.680, 137.680, 265.907
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.060 - 1.550
R-factor	0.129
Rwork	0.129
R-free	0.16140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.650
High resolution limit [Å]	1.550	1.550
Rmerge	0.105	0.970
Rmeas	0.110	1.000
Number of reflections	272024	43832
<I/σ(I)>	11.9	1.8
Completeness [%]	99.9
Redundancy	11
CC(1/2)	0.997	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M Sodium formate; 0.2M Ammonium acetate; 0.2M Sodium citrate tribasic dihydrate; 0.2M Potassium sodium tartrate tetrahydrate; 0.2M Sodium oxamate; 1.0M Imidazole; 1.0M MES monohydrate (acid); 40% v/v Glycerol; 20% w/v PEG 4000