8QA9
Crystal structure of the RK2 plasmid encoded co-complex of the C-terminally truncated transcriptional repressor protein KorB complexed with the partner repressor protein KorA bound to OA-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-14 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.253, 77.095, 84.609 |
| Unit cell angles | 90.00, 107.42, 90.00 |
Refinement procedure
| Resolution | 82.650 - 2.700 |
| R-factor | 0.19402 |
| Rwork | 0.191 |
| R-free | 0.25239 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.774 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.650 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.130 | 1.533 |
| Rmeas | 0.135 | 1.606 |
| Rpim | 0.039 | 0.473 |
| Total number of observations | 45107 | |
| Number of reflections | 29438 | 3879 |
| <I/σ(I)> | 14.1 | 1 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.4 | 11.6 |
| CC(1/2) | 0.993 | 0.677 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | NULL |
