8QA9

Crystal structure of the RK2 plasmid encoded co-complex of the C-terminally truncated transcriptional repressor protein KorB complexed with the partner repressor protein KorA bound to OA-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	173.253, 77.095, 84.609
Unit cell angles	90.00, 107.42, 90.00

Refinement procedure

Resolution	82.650 - 2.700
R-factor	0.19402
Rwork	0.191
R-free	0.25239
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.774
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.650	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.130	1.533
Rmeas	0.135	1.606
Rpim	0.039	0.473
Total number of observations		45107
Number of reflections	29438	3879
<I/σ(I)>	14.1	1
Completeness [%]	100.0
Redundancy	12.4	11.6
CC(1/2)	0.993	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	NULL