8QA8

Crystal structure of the C-terminally truncated transcriptional repressor protein KorB from the RK2 plasmid complexed with CTP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-02
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9179
Spacegroup name	P 21 21 21
Unit cell lengths	58.686, 152.710, 198.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	121.280 - 2.300
R-factor	0.22881
Rwork	0.227
R-free	0.25619
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.441
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	198.270	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.236	1.887
Rmeas	0.245	1.979
Rpim	0.067	0.594
Total number of observations	1069894	50482
Number of reflections	80304	4563
<I/σ(I)>	7.9	0.7
Completeness [%]	100.0
Redundancy	13.3	11.1
CC(1/2)	0.997	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NULL