8Q90
Halophilic Lrp transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.360, 60.000, 87.390 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 43.460 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS (Version Jan 10, 2022) |
| Data scaling software | XSCALE (Version Jan 10, 2022) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.380 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.289 | 1.130 |
| Rpim | 0.108 | |
| Number of reflections | 22193 | 2168 |
| <I/σ(I)> | 4.68 | 1.45 |
| Completeness [%] | 97.9 | 97.13 |
| Redundancy | 7.1 | 7.2 |
| CC(1/2) | 0.979 | 0.679 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2:1 protein:reservoir 0.1 M Sodium acetate pH 3.6 0.2 M Ammonium acetate 10% PEG 4000 20% Ethylene glycol |
