8Q83
Photorhabdus laumondii lectin PLL5 in complex with alpha-methyl-fucoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.712, 102.940, 97.476 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 45.515 - 1.699 |
| Rwork | 0.182 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.262 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.515 | 1.790 |
| High resolution limit [Å] | 1.699 | 1.700 |
| Rmerge | 0.118 | 0.784 |
| Rmeas | 0.138 | 0.917 |
| Rpim | 0.069 | 0.468 |
| Number of reflections | 200282 | 28794 |
| <I/σ(I)> | 6.2 | 1.6 |
| Completeness [%] | 98.8 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.993 | 0.466 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 293 | 20 mM Tris 150 mM NaCl |
