8Q70
tRNA pseudouridine synthase A homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.690, 61.690, 275.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.430 - 1.850 |
| R-factor | 0.1967 |
| Rwork | 0.196 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.655 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_4985) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.430 | 1.878 |
| High resolution limit [Å] | 1.846 | 1.846 |
| Rmerge | 0.175 | 3.114 |
| Number of reflections | 27910 | 1358 |
| <I/σ(I)> | 11.7 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.3 | 22.5 |
| CC(1/2) | 0.997 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.1 M Tris 8.5, 0.2 M MgCl2.6H2O, 10% w/v PEG 8000, 10% w/v PEG 1000 |
