8Q6X
Crystal structure of Cytochrome P450 GymB5 from Streptomyces katrae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.166, 95.544, 81.459 |
| Unit cell angles | 90.00, 109.22, 90.00 |
Refinement procedure
| Resolution | 49.020 - 1.910 |
| R-factor | 0.17096 |
| Rwork | 0.170 |
| R-free | 0.20818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.587 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.020 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.910 |
| Rmeas | 0.120 | |
| Number of reflections | 79072 | 11329 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.8 | |
| CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20 mM sodium formate, 20 mM ammonium acetate, 20 mM sodium citrate, 20 mM potassium sodium tartate, 20 mM sodium oxamate, 13% MPD, 13% PEG 1000, 13% PEG3350, 100 mM BIS-Tris pH 8.7 |
