8Q6Q
Structure of human immunity related GTPase Q in complex with GABARAPL2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 123 |
| Detector technology | PIXEL |
| Collection date | 2021-08-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.100, 64.365, 64.467 |
| Unit cell angles | 60.56, 80.88, 84.77 |
Refinement procedure
| Resolution | 42.220 - 1.800 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.351 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.220 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.034 | 0.479 |
| Number of reflections | 63781 | 3677 |
| <I/σ(I)> | 9.7 | 1.3 |
| Completeness [%] | 88.8 | 86.4 |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 % w/v Polyethylene glycol 3,350, 200 mM di-Ammonium hydrogen citrate; pH 5.0 |
