8Q66

Crystal Structure of the C. elegans MUT-7 MUT-8 CTD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.87313
Spacegroup name	C 1 2 1
Unit cell lengths	145.089, 59.053, 88.161
Unit cell angles	90.00, 119.80, 90.00

Refinement procedure

Resolution	67.910 - 2.030
R-factor	0.1925
Rwork	0.191
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.561
Data reduction software	XDS (20230630)
Data scaling software	pointless
Phasing software	PHENIX (1.12.1-4487)
Refinement software	PHENIX (1.12.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.910	2.103
High resolution limit [Å]	2.030	2.030
Rmerge	0.088
Number of reflections	42083	4152
<I/σ(I)>	9.1
Completeness [%]	99.7
Redundancy	3.4
CC(1/2)	0.997	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	30 %w/v EDO_P8K (Precipitant) 0.1 M MB3 8.5 pH (Buffer) 0.06 M divalent (Complex ingredient)