8Q53
Crystal structure of truncated human Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 43 |
| Unit cell lengths | 60.255, 60.255, 34.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.610 - 1.360 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.610 | 1.409 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.042 | 0.733 |
| Rmeas | 0.046 | 0.794 |
| Rpim | 0.017 | 0.302 |
| Number of reflections | 26382 | 2575 |
| <I/σ(I)> | 21.11 | 2.4 |
| Completeness [%] | 97.1 | 96.12 |
| Redundancy | 6.9 | 6.8 |
| CC(1/2) | 0.999 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 36% PEG 8000, 0.1M acetate pH 4.7 |
