8Q53

Crystal structure of truncated human Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99999
Spacegroup name	P 43
Unit cell lengths	60.255, 60.255, 34.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.610 - 1.360
R-factor	0.1918
Rwork	0.190
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.978
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.610	1.409
High resolution limit [Å]	1.360	1.360
Rmerge	0.042	0.733
Rmeas	0.046	0.794
Rpim	0.017	0.302
Number of reflections	26382	2575
<I/σ(I)>	21.11	2.4
Completeness [%]	97.1	96.12
Redundancy	6.9	6.8
CC(1/2)	0.999	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.7	293	36% PEG 8000, 0.1M acetate pH 4.7