Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 129.560, 129.560, 43.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.360 - 2.150 |
| R-factor | 0.2028 |
| Rwork | 0.200 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.408 |
| Data reduction software | XDS (20230630) |
| Data scaling software | XDS (20230630) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.360 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.313 | 6.080 |
| Rmeas | 0.319 | 6.198 |
| Rpim | 0.062 | 1.197 |
| Number of reflections | 20824 | 2040 |
| <I/σ(I)> | 14.41 | 0.62 |
| Completeness [%] | 99.8 | 98.92 |
| Redundancy | 25.8 | 26.3 |
| CC(1/2) | 0.999 | 0.320 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 30% PEG 4000, 0.1M Tris.HCl, 0.3M Li sulfate |
