8Q4P
Crystal structure of YTHDC1 in complex with Compound 27 (ZA_309)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.850, 103.510, 42.130 |
Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
Resolution | 40.780 - 1.430 |
R-factor | 0.2063 |
Rwork | 0.205 |
R-free | 0.23330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.958 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.781 | 1.510 |
High resolution limit [Å] | 1.430 | 1.430 |
Number of reflections | 59716 | 9327 |
<I/σ(I)> | 7.32 | |
Completeness [%] | 97.3 | |
Redundancy | 3.41 | |
CC(1/2) | 0.990 | 0.763 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5 |