8Q4J

The crystal structure of human chloride intracellular channel protein 5 delta 57-68 F34D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-02
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.886
Spacegroup name	C 2 2 21
Unit cell lengths	129.688, 220.290, 45.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.420 - 2.510
R-factor	0.2428
Rwork	0.241
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.710
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.478	2.660
High resolution limit [Å]	2.505	2.510
Rmeas	0.126	1.975
Number of reflections	22925	3596
<I/σ(I)>	11.8	1.13
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.998	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 7.0, 30% Polyethylene glycol 3,350