8Q4E
Structure of Legionella pneumophila Lcl C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97969 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.600, 52.870, 97.270 |
| Unit cell angles | 90.00, 112.09, 90.00 |
Refinement procedure
| Resolution | 45.560 - 1.900 |
| Rwork | 0.158 |
| R-free | 0.18780 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.180 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.560 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.148 |
| Number of reflections | 34006 | 2460 |
| <I/σ(I)> | 16.7 | 6.7 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.0 M (NH4)2SO4, 0.1 M bis-tris |
