8Q3E

High Resolution Structure of Nucleosome Core with Bound Foamy Virus GAG Peptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98
Detector technology	PIXEL
Collection date	2018-09-03
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.04
Spacegroup name	P 21 21 21
Unit cell lengths	107.168, 109.638, 183.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.852 - 2.174
Rwork	0.234
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.511
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.852	2.290
High resolution limit [Å]	2.170	2.170
Number of reflections	112248	14533
<I/σ(I)>	20.3
Completeness [%]	98.2
Redundancy	12.2
CC(1/2)	1.000	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Buffers containing MnCl2, KCl and K-cacodylate [pH 6.0]