8Q33

Crystal structure of YTHDC1 in complex with Compound 15 (ZA_343)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	40.170, 103.470, 42.120
Unit cell angles	90.00, 104.66, 90.00

Refinement procedure

Resolution	40.750 - 1.430
R-factor	0.2073
Rwork	0.206
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.933
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.750	1.520
High resolution limit [Å]	1.430	1.430
Number of reflections	60090	9521
<I/σ(I)>	14.3
Completeness [%]	97.6
Redundancy	3.444
CC(1/2)	0.999	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5