8Q2X
Crystal structure of YTHDC1 in complex with Compound 10 (ZA_294)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.710, 103.450, 42.120 |
Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
Resolution | 38.210 - 1.600 |
R-factor | 0.2123 |
Rwork | 0.211 |
R-free | 0.24510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.052 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.210 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 41674 | 6615 |
<I/σ(I)> | 9.44 | |
Completeness [%] | 96.6 | |
Redundancy | 3.41 | |
CC(1/2) | 0.996 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5 |