8Q2O
Structure of alginate transporter AlgE from P. aeruginosa PAO1 by using Se-MAG for the the lipid cubic phase crystallization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.090, 74.490, 115.780 |
Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
Resolution | 44.630 - 1.700 |
R-factor | 0.1648 |
Rwork | 0.163 |
R-free | 0.19920 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.661 |
Data reduction software | XDS (VERSION Feb 5, 2021 BUILT=20210323) |
Data scaling software | XSCALE (VERSION Feb 5, 2021 BUILT=20210323) |
Phasing software | SHELXCD (Version 2016/1) |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.630 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmeas | 0.210 | 1.640 |
Number of reflections | 102477 | 7616 |
<I/σ(I)> | 17.63 | 1.61 |
Completeness [%] | 100.0 | 100 |
Redundancy | 45.88 | 9.73 |
CC(1/2) | 0.990 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 293 | 41 %(v/v) PEG400, 100 mM LiSO4, and 0.1 M sodium citrate at pH 5.6 |