8Q1J

LSD1 Y391K-CoREST bound to Acetylated K14 of Histone H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.965459
Spacegroup name	I 2 2 2
Unit cell lengths	118.598, 180.153, 232.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.870 - 2.870
R-factor	0.2409
Rwork	0.240
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.285
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.870	2.920
High resolution limit [Å]	2.840	2.840
Rmerge	0.168	3.855
Rmeas	0.188	4.429
Rpim	0.082	2.114
Total number of observations	279000	13875
Number of reflections	57339	3421
<I/σ(I)>	6.7	0.4
Completeness [%]	97.4
Redundancy	4.9	4.1
CC(1/2)	0.988	0.081

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Sodium Tartate 1.2 M, ADA 0.1M