8Q03
Metagenomic lipase ORF30
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 182.376, 45.742, 135.369 |
Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
Resolution | 46.670 - 1.480 |
R-factor | 0.1457 |
Rwork | 0.144 |
R-free | 0.17980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.714 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.670 | 46.670 | 1.570 |
High resolution limit [Å] | 1.480 | 4.420 | 1.480 |
Rmerge | 0.072 | 0.041 | 0.968 |
Rmeas | 0.082 | 0.048 | 1.173 |
Number of reflections | 159190 | 6719 | 15144 |
<I/σ(I)> | 11.14 | 1.2 | |
Completeness [%] | 88.3 | ||
Redundancy | 15 | ||
CC(1/2) | 0.998 | 0.997 | 0.571 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.6 M ammonium sulfate, 0.1 M Bicine, pH 9.0 |