8PZK
Crystal structure of the Orange Carotenoid Protein 2 (OCP2) from Gloeocapsa sp. PCC 7428
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.661, 52.661, 216.831 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.208 - 1.800 |
| Rwork | 0.197 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.819 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.280 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.134 | 2.668 |
| Rmeas | 0.153 | 3.031 |
| Rpim | 0.072 | 1.414 |
| Total number of observations | 125097 | 6915 |
| Number of reflections | 29081 | 1584 |
| <I/σ(I)> | 6.6 | 0.7 |
| Completeness [%] | 98.2 | |
| Redundancy | 4.3 | 4.4 |
| CC(1/2) | 0.996 | 0.283 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.2 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.0, 18% w/v Polyethylene glycol monomethyl ether 5,000 |
