8PZ3
TssM - A USP-like DUB from B. pseudomallei (193-430)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.850, 116.850, 111.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.600 - 3.150 |
| R-factor | 0.2336 |
| Rwork | 0.229 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.946 |
| Data reduction software | XDS (BUILT=20210323) |
| Data scaling software | XSCALE (BUILT=20210323) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18rc4_3812) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.880 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmeas | 0.250 | 1.079 |
| Number of reflections | 15249 | 1109 |
| <I/σ(I)> | 5.75 | 1.53 |
| Completeness [%] | 97.6 | 96.9 |
| Redundancy | 4.69 | 4.76 |
| CC(1/2) | 0.985 | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.15 M ammonium chloride, 22 % w/v PEG 3350 and 4 % Dextran sulfate sodium salt Mr 5,000; 1:2, 1:1, 2:1 protein reservoir drop ration; cryoprotected with reservoir + 25% Glycerol |
