8PZ0
Intracellular leucine aminopeptidase of Pseudomonas aeruginosa PA14.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 181.950, 181.950, 87.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.490 - 1.800 |
| R-factor | 0.1687 |
| Rwork | 0.168 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.897 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.490 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 4.030 | |
| Rmeas | 0.140 | 4.141 |
| Number of reflections | 78846 | 3880 |
| <I/σ(I)> | 17.5 | 0.8 |
| Completeness [%] | 100.0 | 99.59 |
| Redundancy | 36.7 | 18.56 |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 14 % PEG 3350 and 200 mM ammonium nitrate |
