8PXH
Structure of TauA from E. coli, solved at wavelength 2.375 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-08-03 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.375 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.010, 71.320, 165.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.510 - 1.770 |
| R-factor | 0.20312 |
| Rwork | 0.202 |
| R-free | 0.23145 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.527 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.510 | 1.833 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.134 | 0.878 |
| Rmeas | 0.138 | |
| Rpim | 0.030 | |
| Number of reflections | 45258 | 3837 |
| <I/σ(I)> | 15.63 | 2.65 |
| Completeness [%] | 85.7 | 73.52 |
| Redundancy | 19.4 | 16.9 |
| CC(1/2) | 0.997 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.25 M sodium iodide, 0.1 M Bis-Tris propane, 24% (w/v) PEG 3350 + 10 mM ACES |
