8PXH

Structure of TauA from E. coli, solved at wavelength 2.375 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I23
Synchrotron site	Diamond
Beamline	I23
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-08-03
Detector	DECTRIS PILATUS 12M
Wavelength(s)	2.375
Spacegroup name	P 21 21 21
Unit cell lengths	45.010, 71.320, 165.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.510 - 1.770
R-factor	0.20312
Rwork	0.202
R-free	0.23145
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.527
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.510	1.833
High resolution limit [Å]	1.770	1.770
Rmerge	0.134	0.878
Rmeas	0.138
Rpim	0.030
Number of reflections	45258	3837
<I/σ(I)>	15.63	2.65
Completeness [%]	85.7	73.52
Redundancy	19.4	16.9
CC(1/2)	0.997	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.25 M sodium iodide, 0.1 M Bis-Tris propane, 24% (w/v) PEG 3350 + 10 mM ACES