8PXE
Crystal structure of the N-terminal dimerisation domain of Hh1141
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.004, 66.004, 113.245 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.670 - 3.230 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.664 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.1_3469) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.670 | 46.670 | 3.430 |
High resolution limit [Å] | 3.230 | 9.520 | 3.230 |
Rmerge | 0.170 | 0.045 | 1.732 |
Rmeas | 0.178 | 0.048 | 1.804 |
Number of reflections | 4360 | 205 | 675 |
<I/σ(I)> | 14.75 | ||
Completeness [%] | 99.8 | ||
Redundancy | 12.14 | ||
CC(1/2) | 0.997 | 0.997 | 0.611 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PACT F8 | 0.2 M sodium sulfate, 0.1 M Bis Tris propane 6.5, 20 % w/v PEG 3350. 30% EDO used as cryoprotectant. |