8PXD

Crystal structure of the C-terminal domain of Hh1412

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	I 2 2 2
Unit cell lengths	37.700, 64.480, 119.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.954 - 2.200
R-factor	0.1944
Rwork	0.191
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.061
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MoRDa
Refinement software	PHENIX ((1.15.1_3469: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.960	35.960	2.220
High resolution limit [Å]	2.090	6.200	2.090
Rmerge	0.210	0.143	1.324
Rmeas	0.219	0.149	1.383
Number of reflections	8910	393	1378
<I/σ(I)>	6.41		1.13
Completeness [%]	99.6		97.6
Redundancy	12.38		11.85
CC(1/2)	0.993	0.992	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Morpheus A4; 0.06 M Divalents, 0.1 M Buffer System 1 6.5, 50 % v/v Precipitant Mix 4