8PX7
Structure of Bacterial Multidrug Efflux transporter AcrB, solved at wavelength 3.02 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-10-12 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 3.024 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 145.300, 145.300, 516.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.940 - 3.400 |
| R-factor | 0.302 |
| Rwork | 0.301 |
| R-free | 0.31000 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.940 | 3.521 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.160 | 0.160 |
| Rmeas | 0.160 | |
| Rpim | 0.017 | 0.017 |
| Number of reflections | 29410 | 2892 |
| <I/σ(I)> | 31.15 | 0.97 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 86.9 | 73.1 |
| CC(1/2) | 1.000 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 4000, 0.1 M HEPES PH 7.5, 0.1 M AMMONIUM SULPHATE AND 22% V/V GLYCEROL |
