8PWI

Light structure of sensory rhodopsin-II solved by serial millisecond crystallography 60-90 milliseconds time-bin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-05-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000342
Spacegroup name	C 2 2 21
Unit cell lengths	89.750, 131.700, 51.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.880 - 1.960
R-factor	0.2462
Rwork	0.245
R-free	0.27630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.794
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.900	44.000
High resolution limit [Å]	1.960	2.500
Number of reflections	4429	12660
<I/σ(I)>	5.22
Completeness [%]	99.5
Redundancy	72.06
CC(1/2)	0.983	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		296	CaCl2 150mM, Glycine 100 mM, 38% (v/v) PEG 400, pH 7.5