8PVW
Structure of a short E. coli adenylate kinase in complex with Ap5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-08 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 30.838, 73.244, 90.357 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.450 - 2.000 |
| R-factor | 0.1859 |
| Rwork | 0.183 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.725 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.450 | 2.500 |
| High resolution limit [Å] | 1.700 | 1.955 |
| Rmerge | 0.092 | |
| Rpim | 0.036 | |
| Number of reflections | 13571 | 13571 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 78.7 | |
| Redundancy | 10 | |
| CC(1/2) | 0.988 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 30% PEG 4000, 0.2 M Ammonium sulfate |
