8PV0

Crystal structure of tropomyosin (Cdc8) cables, Conformers 2 and 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9797
Spacegroup name	P 1 21 1
Unit cell lengths	46.499, 77.667, 108.224
Unit cell angles	90.00, 94.20, 90.00

Refinement procedure

Resolution	53.970 - 2.430
R-factor	0.2546
Rwork	0.251
R-free	0.31060
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.374
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PARROT
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.930	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.088
Number of reflections	28799	2178
<I/σ(I)>	9.8
Completeness [%]	98.9
Redundancy	8.9
CC(1/2)	0.887	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.8	298	10 mM Tris HCl pH 7.8, 0.15 M ammonium acetate, and 40% MPD