8PUL
ChiLob 7/4 H2 HC-K223C/C224S Kappa LC-C214S F(ab')2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 45 |
| Detector technology | PIXEL |
| Collection date | 2022-05-25 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.7552 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.995, 95.818, 151.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.177 - 1.930 |
| Rwork | 0.203 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.794 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 151.920 | 1.970 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.058 | 1.879 |
| Rmeas | 0.061 | 2.025 |
| Rpim | 0.018 | 0.729 |
| Number of reflections | 71110 | 3876 |
| <I/σ(I)> | 16.93 | 0.283 |
| Completeness [%] | 85.7 | 73.7 |
| Redundancy | 9.58 | 7.3 |
| CC(1/2) | 0.997 | 0.274 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | 0.1M TRIS, 15% PEG 4000, 15% glycerol |
