8PU1
Structure of the toxin/antitoxin complex FaRel/ATfaRel2 with APCPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2021-12-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 227.810, 227.810, 227.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.260 - 1.702 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.860 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (24-FEB-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.260 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 55679 | 78533 |
| <I/σ(I)> | 24.47 | |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 68.4 | |
| CC(1/2) | 1.000 | 0.399 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3% v/v 2-Propanol and 0.1 M Sodium, pH 6.5 |
