8PS7
Crystal structure of Medicago truncatula LYR4 kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-26 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979491 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.684, 78.242, 70.365 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 43.120 - 2.100 |
| R-factor | 0.225 |
| Rwork | 0.221 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.479 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.124 | 2.340 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.240 | |
| Number of reflections | 18580 | 929 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 91.2 | 70.2 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.980 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | ammonium sulfate, sodium cacodylate trihydrate, PEG8000 |
